Used applied biosystems abi prism 310 genetic analyzer. Send us your comments applied biosystems welcomes your. Page 214 any other user with the scientist role specified and electronic signature enabled in their user account if a user that does not meet the specified criteria signs, a message is displayed to indicate which users have esigned. Rather than risking inconsistency as a result of pouring slabgels, a simple capillary array is fit into the slots. Paternity testing and forensic dna typing by multiplex str. Note many abi p rism 310 genetic analyzer users have already received the abice2 basecaller separately from the sequencing analysis application. This is the user s manual for the abi prism 310 genetic analyzer with a macintosh computer. This information applies to the syringes manufactured by the kloehn company. Powerplexr 5c matrix standards, 310 technical manual. In addition to precision and flexibility, this platform offers exceptional convenience, with full automation for every procedural step from gel loading to data acquisition and analysis.
Abi genetic analyzer usage at nist all instruments were purchased using nij funds abi 310 1st was purchased in 1996 as mac a230, now b233 2nd was purchased in june 2002 as nt b261 abi 3100 3xl 1st purchased in april 2001 as abi 3100 upgraded to 3xl in sept 2005 located in a different room a230, now b219. Cib forensic science center training seminar june 67, 2012. Abi prism 310 genetic analyzer user guide thermo fisher. Pdf paternity testing and forensic dna typing by multiplex. Applied biosystems 37303730 xl our scientific research. Abi prism 310 genetic analyzer applied biosystems laboratoryautomation molecular biology laboratories need technology useyet sufficiently flexible need,applied biosystems built upon its proven multicolor fluorescent labeling technology completelyautomated, yet flexible system sequence,size, quantitatenucleic acids. L of genescan 500 liz size standard applied biosystems. Seqstudio genetic analyzer is a lowthroughput, easytouse, and convenient benchtop system that delivers goldstandard sanger sequencing technology and fragment analysis with just a simple click. Paternity testing and forensic dna typing by multiplex str analysis using abi prism 310 genetic analyzer article pdf available june 2012 with 1,918 reads how we measure reads. Place the fast plate adapter on the autosampler tray holder. Identifiler analysis on the abi 3xl genetic analyzer a. Applied biosystems 310 genetic analyzer user guide. Perkin elmer abi prism 310 genetic analyzer for sale online.
About the kit the abi prism snapshot multiplex kit is designed to interrogate refer to the abi prism 310 genetic analyzer users manual pn 903565. The abi prism 3100 and 3100avant genetic analyzer includes patented technologylicensed from hitachi, ltd. Applied biosystems 37303730xl dna analyzers user guide pn. Applied biosystems 310 genetic analyzer applied biosystems 3500 series genetic analyzer applied biosystems 3 series genetic analyzer applied biosystems 3100 series genetic analyzer. Attachments abi prism 310 genetic analyzer manual 3 mb. The abi prism 310 analyzer windowscompatible system allows data to be easily transferred between different platforms such as the abi prism 3100 and 3100avant genetic analyzers as well as to downstream analysis software, the industry standards for automated genotyping, sequencing,and mutation analysis. They performed sensitivity and mixture studies using control dna samples and compared typing results in a limited set of casework samples previously typed with the yfiler kit. The use of powerplex y23 system with the abi prism 310. Automated fluorescent dna sequencing on the abi prism 310 genetic analyzer.
Abi genetic analyzer the abi prism 310 genetic analyzer is an automated singlecapillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications. Validation of the ampflstr ngm select pcr amplification kit. These machines use capillary electrophoresis, which is a significant advantage over slabgel based technologies. For more information about when to use the abice2 basecaller, see ochoosing a basecallero on page 544 and othe abi basecallerso on page 544. Applied biosystems 310 genetic analyzer data collection software preferences module windows nt, 2000, and xp os.
Page 1 applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. For additional documentation, see how to obtain support on page vii. Prism 310 genetic analyzer manual conquer scientific. Abi prism 310 genetic analyzer a benchmark in laboratory automation molecular biology laboratories need technology that is easy to use yet sufficiently flexible for a variety of applications. Capillary electrophoresis ce instruments, such as the abi prism 310 and abi 3100 genetic analyzers, are the method of choice for many laboratories performing str analysis. Identifiler analysis on the abi 3xl genetic analyzer. Abi prism 3100 genetic analyzer abi prism 3100avant genetic analyzer abi prism 310 genetic analyzer abi prism 377 dna sequencer all models general instructions are given for using the kit reagents to generate samples for these instruments. Forensic dna typing by capillary electrophoresis using the. For macintosh os refer to the abi prism 310 genetic analyzer. Abi p rism 310 genetic analyzer users manual pn 903565 genescan analysis software users manual pn 904435.
Abi 310 sequencer the abi prism 310 genetic analyzer is an automated singlecapillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications. Information contained in this posting is as known to us. Applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Applied biosystems built upon its proven multicolor fluorescent labeling technology to achieve a completely automated, yet flexible system to sequence, size, and quantitate nucleic acids.
One microliter of each pcr reaction product was added to 24. For more detailed instructions, refer to the appropriate instrument users. The 310 genetic analyzer is the standard in laboratory convenience. Fbi klein, wallin, and buoncristiani, addressing ambient. Surplus property is not tested unless otherwise stated in description. The authors of this article report the successful analysis of amplicons obtained with the powerplex y23 system on the abi prism 310 genetic analyzer. Open the abi prism 310 data collection software, version 3. Abi p rism 310 genetic analyzer user s manual pn 903565 genescan analysis software user s manual pn 904435 introduction technical support to reach us on the web applied biosystems web site address is. The abi prism 3100 genetic analyzer includes patented technology licensed from hitachi, ltd. To meet this need, applied biosystems built upon its proven multicolor fluorescent labeling technology to achieve a completely automated. For windows nt os refer to the abi prism 310 genetic analyzer. Abi prism 310 genetic analyzer user guide describes the abi prism 310 genetic analyzer hardware and software and provides information on preparing, maintaining, and troubleshooting the system. Perform comparative sequencing, linkage analysis, str analysis, snp detection, discovery and validation, mutation detection, and many other applications.
Installing the genemapper software and creating a matrix on the abi prism 310 genetic analyzer in this user bulletin this user bulletin covers the following topics. Select module fast gs template a or fast gs template d from the module popup menu for every. A single capillary sequencer, abi prism 310, has proven its admirable longterm reliability and excellent performance and is very popular among its users. Abi prism 3100 genetic analyzer boyce thompson institute. After samples are placed in the sample tray, virtually all aspects of analysis are automated. The system achieves unparalleled ease of use through the integration of abi prism.
Applied biosystems seqstudio genetic analyzer thermo. User bulletin national forensic science technology center. The abi prism 310 genetic analyzer is one of the more commonly used dna analysis instruments in forensic laboratories. Automated fluorescent dna sequencing on the abi prism 310. This is the users manual for the abi prism 310 genetic analyzer with a macintosh computer. User should be administrator, password should be left blank. For information about the power, refer to the abi prism 310 genetic analyzer site. Standard 96well plates cannot be used on the abi p rism 310 genetic analyzer. Pe biosystems introduces abi prism 3700 dna analyzer for. The amplified fragments were analyzed with the abi prism 310 genetic analyzer applied biosystems. Applied biosystems abi prism 310 genetic analyzer inquire about availability computer is loaded with windows xp, ab sequencing analysis software v5. Dna synthesis, fluorescencebased dna analysis, and the polymerase chain reaction pcr are being integrated for a range of applications, from forensics to the understanding and treatment of genetic disease.
David fegredo medical scientistpathwestaustralia the abi 3 and 3xl genetic analyzers are the newest in a range of analyzers produced by abi. This chapter provides detailed instructions for performing sequencing on the abi prism 310 genetic analyzer, using pop6, running version 1. Applied biosystems 35003500xl genetic analyzer user guide pn. Applied biosystems 3500 user manual pdf download manualslib. L of deionized formamide sigma, st louis, mo, usa and 0.
Instrument setup is quick and easy, with a simple read more. Three fundamental technologies have emerged in genetic analysis that have widespread and immediate benefits. Applied biosystems 33xl genetic analyzers quick reference card pn 4362825 contains a flowchart on how to run the samples and instrument, a table of maintenance tasks, and a data collection software reference guide. Dec 20, 2006 abi prism 310 genetic analyzer the fast plate adapter for the abi prism 310 genetic analyzer is designed to hold only the fast 96well plate. Genescan reference guide chemistry reference for the abi p. The ampflstr ngm select user guide describes instrument setup and sample preparation of pcr products using the abi prism 3100 and 3100avant genetic analyzers and applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers, but not for abi prism 310 genetic analyzer, sequencer still widely used in many forensic laboratories. It is an automated, singlecapillary electrophoresis instrument with laser. Abi p rism 310 genetic analyzer user guide draft june 21, 2001 10.
The abi prism 310 genetic analyzer is the smaller scale solution for dna analysis. Perkin elmer abi prism 310 genetic analyzer for sale. Applied biosystems 310 genetic analyzer support thermo. Available as an upgrade to an existing 3100 or as a factory. Refer to the abi prism 310 genetic analyzer users manual for instructions on cleaning the pump block, installing the capillary, calibrating the autosampler. Applied biosystems abi prism 310 genetic analyzer biosurplus. Syringe care and maintenance overview this user bulletin describes the care, use, and maintenance of the syringes used with the abi p rism 310 genetic analyzer.
Abi genetic analyzer usage at nist all instruments were purchased using nij funds abi 310 1st was purchased in 1996 as mac a230, now b233 2nd was purchased in june 2002 as nt b261 abi 3100. Applied biosystems abi prism 310 genetic analyzer sequencer. Cib forensic science center training seminar june 67. Applied biosystems 37303730xl dna analyzers user guide iii. Upgrade of applied biosystems genetic analyzer, model abi prism 310, to windows 10. Abi prism 310 genetic analyzer thermo fisher scientific. Refer to the abi prism 310 genetic analyzer users manual for instructions on cleaning the pump block, installing the capillary, calibrating the autosampler and adding polymer to the syringe. There is no need to pour conventional polyacrylamide and agarose slab gels or to load samples for electrophoresis. Select the appropriate sample sheet from the sample sheet popup menu. Used applied biosystems abi prism 310 genetic analyzer, model. Errors encountered on the applied biosystems 310 genetic analyzer. The abi prism 310 by applied biosystems is a genetic analyzing system designed to sequence, size, and quantitate nucleic acids. Ideal for the lower throughput needs of individuals laboratories, the 310 analyzer is convenient and easy to operate, combining the same capillary electrophoresis and walkaway automation of the 3700 analyzer. Page 8 of 16 abi prism 310 genetic analyzer 5 set up the injection list as described in the abi prism 310 genetic analyzer users manual.